You choose whether to add or remove a counter when the second ability resolves. You can't choose to remove a counter if there isn't one there.
If the third ability triggers, removing a counter in response won't stop the effect. However, somehow removingPlague Boilerfrom the battlefield in response would stop the effect because then you wouldn't be able to sacrificePlague Boiler.
The sacrifice is done on resolution of the triggered ability.
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